Recombinant Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088] (ab308034) (2024)

Overview

• Product name

  Anti-EPO-R (phospho Y454 + Y456) antibody [RP23040088]
  See all EPO-R primary antibodies

• Description

  Rabbit recombinant multiclonal [RP23040088] to EPO-R (phospho Y454 + Y456)

• Host species

  Rabbit

• Tested applications

  Suitable for: WB, ICC/IFmore details

• Species reactivity

  Reacts with: Human
  

• Immunogen

  Synthetic peptide corresponding to Human EPO-R (phospho Y454 + Y456).
  Database link: P19235

• Positive control

  • WB: HeLa and K-562 cell lysates.ICC/IF: HeLa cells.

• General notes

  Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

  See Also

  L'Oréal Paris Colorista Washout - Hotpink - 1-2 weken Haarkleuring | bolCreate and manage rules | Kibana Guide [8.13]

  Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Properties

• Form

  Liquid

• Storage instructions

  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

• Storage buffer

  Preservative: 0.09% Sodium azide
  Constituent: 99.91% PBS

• Concentration information loading...

• Purity

  Protein A purified

• Clonality

  Recombinant Multiclonal

• Clone number

  RP23040088

• Isotype

  IgG

• Research areas

  • Immunology
  • Immunoglobulins
  • Receptors

  • Signal Transduction
  • Signaling Pathway
  • G Protein Signaling
  • Small G Proteins
  • Other

  • Cardiovascular
  • Angiogenesis
  • Angiogenic Factors

  • Stem Cells
  • Hematopoietic Progenitors
  • Surface Molecules

  • Cardiovascular
  • Atherosclerosis
  • Ischemia / Reperfusion

  • Cardiovascular
  • Heart
  • Apoptosis

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Cardiovascular
  • Angiogenesis
  • Endothelial Cell Markers

Applications

Target

• Function

  Receptor for erythropoietin. Mediates erythropoietin-induced erythroblast proliferation and differentiation. Upon EPO stimulation, EPOR dimerizes triggering the JAK2/STAT5 signaling cascade. In some cell types, can also activate STAT1 and STAT3. May also activate the LYN tyrosine kinase.
  Isoform EPOR-T acts as a dominant-negative receptor of EPOR-mediated signaling.

• Tissue specificity

  Erythroid cells and erythroid progenitor cells. Isoform EPOR-F is the most abundant form in EPO-dependent erythroleukemia cells and in late-stage erythroid progenitors. Isoform EPOR-S and isoform EPOR-T are the predominant forms in bone marrow. Isoform EPOR-T is the most abundant from in early-stage erythroid progenitor cells.

• Involvement in disease

  Defects in EPOR are the cause of erythrocytosis familial type 1 (ECYT1) [MIM:133100]. ECYT1 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin and hematocrit, hypersensitivity of erythroid progenitors to erythropoietin, erythropoietin low serum levels, and no increase in platelets nor leukocytes. It has a relatively benign course and does not progress to leukemia.

• Sequence similarities

  Belongs to the type I cytokine receptor family. Type 1 subfamily.
  Contains 1 fibronectin type-III domain.

• Domain

  The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
  The box 1 motif is required for JAK interaction and/or activation.
  Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.

• Post-translational
  modifications

  On EPO stimulation, phosphorylated on C-terminal tyrosine residues by JAK2. The phosphotyrosine motifs are also recruitment sites for several SH2-containing proteins and adapter proteins which mediate cell proliferation. Phosphorylation on Tyr-454 is required for PTPN6 interaction, Tyr-426 for PTPN11. Tyr-426 is also required for SOCS3 binding, but Tyr-454/Tyr-456 motif is the preferred binding site.
  Ubiquitinated by NOSIP; appears to be either multi-monoubiquitinated or polyubiquitinated. Ubiquitination mediates proliferation and survival of EPO-dependent cells.

• Cellular localization

  Cell membrane and Secreted. Secreted and located to the cell surface.

• Information by UniProt

• Database links

  • Entrez Gene: 2057 Human
  • Omim: 133171 Human
  • SwissProt: P19235 Human
  • Unigene: 631624 Human

• Alternative names

  • EPO R antibody
  • EPO Receptor antibody
  • EPO-R antibody

  see all

Images

Protocols

Datasheets and documents

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